New enzyme belonging to the family of molybdenum-free nitrate reductases1

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New enzyme belonging to the family of molybdenum-free nitrate reductases.

A novel molybdenum-free nitrate reductase was isolated from the obligate chemolithoautotrophic and facultative anaerobic, (halo)alkaliphilic sulphur-oxidizing bacterium Thioalkalivibrio nitratireducens strain ALEN 2. The enzyme was found to contain vanadium and haem c as cofactors. Its native molecular mass was determined as 195 kDa, and the enzyme consists of four identical subunits with appar...

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Molybdenum and Nitrate Reductase

Numerous investigators have shown that nitrate nitrogen accumulates in higher plants (l-3) and fungi (2, 4) which are deficient in molybdenum. Other workers have shown that sulfur, zinc, and manganese deficiencies in higher plants may also result in a similar phenomenon (5). The free amino acids present in molybdenum-deficient plants are usually less than those in normal tissues, thus indicatin...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 2003

ISSN: 0264-6021,1470-8728

DOI: 10.1042/bj20021193